3D6K
The crystal structure of a putative aminotransferase from Corynebacterium diphtheriae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97943 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.248, 56.081, 173.592 |
| Unit cell angles | 90.00, 102.47, 90.00 |
Refinement procedure
| Resolution | 42.680 - 2.000 |
| R-factor | 0.19249 |
| Rwork | 0.190 |
| R-free | 0.24093 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.546 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.700 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.084 | 0.377 |
| Number of reflections | 99497 | |
| <I/σ(I)> | 12 | 1.45 |
| Completeness [%] | 86.9 | 64.7 |
| Redundancy | 2.9 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2M NH4Cl 20% (w/v) PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
