3D6I
Structure of the Thioredoxin-like Domain of Yeast Glutaredoxin 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.906, 48.611, 55.294 |
| Unit cell angles | 90.00, 116.76, 90.00 |
Refinement procedure
| Resolution | 49.390 - 1.500 |
| R-factor | 0.225 |
| Rwork | 0.223 |
| R-free | 0.24900 |
| Starting model (for MR) | 1ert |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.302 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.062 | 0.347 |
| Number of reflections | 35163 | |
| <I/σ(I)> | 18.7 | |
| Completeness [%] | 95.9 | 69.1 |
| Redundancy | 3.5 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 0.1M MOPS, 20mM 2-ME, 68% ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
