3D6C
Crystal structure of Staphylococcal nuclease variant PHS L38E at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-01 |
| Detector | APEX II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 |
| Unit cell lengths | 48.256, 48.256, 62.675 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.280 - 2.000 |
| R-factor | 0.179 |
| Rwork | 0.173 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.591 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.300 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.022 | 0.110 |
| Number of reflections | 9781 | |
| <I/σ(I)> | 40.02 | 8.31 |
| Completeness [%] | 99.8 | 98.9 |
| Redundancy | 13.82 | 5.88 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 35% MPD, 25 mM Potassium Phosphate, Calcium chloride, PTP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
