3D69

Crystal Structure of the Fab Fragment of an Anti-Factor IX Antibody 10C12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2004-09-12
Spacegroup name	P 41
Unit cell lengths	159.609, 159.609, 65.366
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.260 - 3.770
Rwork	0.249
R-free	0.34200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nl0
RMSD bond length	0.009
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.770
Rmerge	0.217
Number of reflections	13950
<I/σ(I)>	141.4
Completeness [%]	82.2
Redundancy	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	3.0M ammonium sulfate, 0.1M HEPES, 2%MPD, pH7.5, VAPOR DIFFUSION, temperature 293K