3D69
Crystal Structure of the Fab Fragment of an Anti-Factor IX Antibody 10C12
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-09-12 |
Spacegroup name | P 41 |
Unit cell lengths | 159.609, 159.609, 65.366 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.260 - 3.770 |
Rwork | 0.249 |
R-free | 0.34200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nl0 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.770 |
Rmerge | 0.217 |
Number of reflections | 13950 |
<I/σ(I)> | 141.4 |
Completeness [%] | 82.2 |
Redundancy | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 293 | 3.0M ammonium sulfate, 0.1M HEPES, 2%MPD, pH7.5, VAPOR DIFFUSION, temperature 293K |