3D5J
Structure of yeast Grx2-C30S mutant with glutathionyl mixed disulfide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2006-08-10 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.427 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.008, 45.205, 105.035 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.900 - 1.910 |
| R-factor | 0.201 |
| Rwork | 0.198 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.434 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.010 | 2.010 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Number of reflections | 14326 | |
| <I/σ(I)> | 23.6 | 4.5 |
| Completeness [%] | 99.7 | 98.3 |
| Redundancy | 8.6 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 298 | PEG 4000, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
