3D5G
Structure of ribonuclease Sa2 complexes with mononucleotides: new aspects of catalytic reaction and substrate recognition
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-09-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.655, 67.667, 57.085 |
| Unit cell angles | 90.00, 100.63, 90.00 |
Refinement procedure
| Resolution | 11.980 - 1.800 |
| R-factor | 0.1771 |
| Rwork | 0.174 |
| R-free | 0.24323 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pyl |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.892 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 1.820 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.037 | 0.240 |
| Number of reflections | 34199 | |
| <I/σ(I)> | 32.16 | |
| Completeness [%] | 97.7 | 74.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | 30% ammonium sulphate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
