3D54
Structure of PurLQS from Thermotoga maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 256.851, 187.337, 159.177 |
| Unit cell angles | 90.00, 99.12, 90.00 |
Refinement procedure
| Resolution | 45.700 - 3.500 |
| R-factor | 0.252 |
| Rwork | 0.252 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.700 | 3.720 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 78498 | |
| Completeness [%] | 83.8 | 66.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 55% MPD, 0.1M HEPES, 7% xylitol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
