3D3Y
Crystal structure of a conserved protein from Enterococcus faecalis V583
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | I 4 |
| Unit cell lengths | 122.437, 122.437, 69.003 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.720 - 1.950 |
| R-factor | 0.17446 |
| Rwork | 0.172 |
| R-free | 0.21285 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.395 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.720 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.083 | 0.581 |
| Number of reflections | 37069 | |
| <I/σ(I)> | 28.5 | 1.8 |
| Completeness [%] | 99.6 | 95.2 |
| Redundancy | 4.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 289 | 0.1M Sodium acetate, 4M Ammonium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
