3D3R
Crystal structure of the hydrogenase assembly chaperone HypC/HupF family protein from Shewanella oneidensis MR-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97921 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.864, 58.316, 51.924 |
| Unit cell angles | 90.00, 127.89, 90.00 |
Refinement procedure
| Resolution | 38.750 - 1.850 |
| R-factor | 0.203 |
| Rwork | 0.202 |
| R-free | 0.23400 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.851 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.750 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.102 | 0.328 |
| Number of reflections | 15804 | |
| <I/σ(I)> | 10.1 | 3.92 |
| Completeness [%] | 98.6 | 100 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 20% PEG 3350, 0.2M Ammonium citrate, 0.3M NDSB211, 0.5mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
