3D3D

Bacteriophage lambda lysozyme complexed with a chitohexasaccharide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-08-30
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.502
Spacegroup name	P 21 21 21
Unit cell lengths	52.200, 61.081, 122.447
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.940 - 2.600
Rwork	0.234
R-free	0.27700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1am7
RMSD bond length	0.009
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.942	2.760
High resolution limit [Å]	2.600	2.600
Rmerge	0.118	0.507
Number of reflections	11801
<I/σ(I)>	7.9
Completeness [%]	92.0	51.9
Redundancy	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	4.6	298	0.1 M NaOAc pH 4.6, 0.1 M ammonium sulfate, 20% w/v PEG 2000 MME, microdialysis, temperature 298K