3D38

Crystal structure of new trigonal form of photosynthetic reaction center from Blastochloris viridis. Crystals grown in microfluidics by detergent capture.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-16
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0332
Spacegroup name	P 31 2 1
Unit cell lengths	241.171, 241.171, 113.391
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 3.210
R-factor	0.194
Rwork	0.192
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2i5n
RMSD bond length	0.012
RMSD bond angle	1.437
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.4.0073)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.200
Rmerge	0.168
Number of reflections	62037
<I/σ(I)>	30.3
Completeness [%]	99.8
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Plug-based Microfluidics	6	296	9 mg/mL Protein, 4.6 mM LDAO, 10 mM Methyl-beta-cyclodextrin, 1.6-1.7 M Sulfate, 4.5% w/v Triethylammonium phosphate pH 6.0, 3% 1,2,3-heptanetriol, 30 mM NaH2PO4-Na2HPO4 pH 6.0, Plug-based Microfluidics, temperature 296K