3D31
ModBC from Methanosarcina acetivorans
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 293.15 |
| Detector technology | PIXEL |
| Collection date | 2007-09-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9537, 0.9789, 1.2143, 1.2147 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 92.840, 139.460, 207.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| Rwork | 0.251 |
| R-free | 0.28200 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.607 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELX |
| Refinement software | CNS (v. 1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.140 | |
| Number of reflections | 42405 | |
| <I/σ(I)> | 14.4 | |
| Completeness [%] | 77.8 | |
| Redundancy | 19.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293.15 | 18% (w/v) PEG 3350, 200mM magnesium formate, 50mM Tris-HCl pH 8.0, VAPOR DIFFUSION, temperature 293.15K |
