3D1J
Crystal Structure of E.coli GS mutant dmGS(C7S;C408S)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 32-ID |
Synchrotron site | APS |
Beamline | 32-ID |
Temperature [K] | 98 |
Detector technology | CCD |
Collection date | 2004-10-16 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 2 |
Unit cell lengths | 232.959, 232.959, 85.539 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 3.000 |
R-factor | 0.212 |
Rwork | 0.209 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rzu |
RMSD bond length | 0.012 |
RMSD bond angle | 1.469 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.150 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.076 | 0.345 |
Number of reflections | 20539 | |
<I/σ(I)> | 28.3 | 2 |
Completeness [%] | 99.2 | 100 |
Redundancy | 9.4 | 9.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 40% (w/v) PEG 4000, 0.1 M Tris, 0.2 M Na tartrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |