3D0X
Crystal Structure of the unbound lysine riboswitch
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-02-26 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 |
| Unit cell lengths | 120.193, 120.193, 58.248 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 17.112 - 2.950 |
| R-factor | 0.1907 |
| Rwork | 0.187 |
| R-free | 0.22030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3D0U without lysine |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.050 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.700 | 3.060 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.090 | 0.355 |
| Number of reflections | 19610 | |
| <I/σ(I)> | 10.2 | 3.4 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 3.62 | 3.63 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LITHIUM SULFATE, MAGNESIUM CHLORIDE, IRIDIUM HEXAMMINE, SODIUM HEPES |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | LITHIUM SULFATE | ||
| 2 | 1 | 1 | MAGNESIUM CHLORIDE | ||
| 3 | 1 | 1 | IRIDIUM HEXAMMINE | ||
| 4 | 1 | 1 | SODIUM HEPES | ||
| 5 | 1 | 2 | LITHIUM SULFATE | ||
| 6 | 1 | 2 | MAGNESIUM CHLORIDE | ||
| 7 | 1 | 2 | IRIDIUM HEXAMMINE | ||
| 8 | 1 | 2 | SODIUM HEPES |
