3D0W
Crystal structure of YflH protein from Bacillus subtilis. Northeast Structural Genomics Consortium target SR326
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-28 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.300, 45.180, 99.177 |
| Unit cell angles | 90.00, 98.27, 90.00 |
Refinement procedure
| Resolution | 33.240 - 2.000 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.24200 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.063 | 0.335 |
| Number of reflections | 57564 | |
| <I/σ(I)> | 15.2 | 14 |
| Completeness [%] | 99.3 | 97.6 |
| Redundancy | 3.8 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | 0.1M Citric acid, 30% PEG 6000, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | 0.1M Citric acid, 30% PEG 6000, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
