3D0J
Crystal structure of conserved protein of unknown function CA_C3497 from Clostridium acetobutylicum ATCC 824
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 52.878, 52.878, 112.280 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.790 - 1.530 |
| R-factor | 0.166 |
| Rwork | 0.165 |
| R-free | 0.18400 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.719 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.790 | 1.570 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Number of reflections | 27647 | |
| <I/σ(I)> | 12.1 | 3.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 13.8 | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 289 | 3.5M Sodium formate, 0.1M Sodium acetate pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
