3CZX
The crystal structure of the putative N-acetylmuramoyl-L-alanine amidase from Neisseria meningitidis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.955, 65.039, 79.319 |
| Unit cell angles | 90.00, 109.53, 90.00 |
Refinement procedure
| Resolution | 74.740 - 1.600 |
| R-factor | 0.18201 |
| Rwork | 0.180 |
| R-free | 0.21779 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.444 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.740 | 1.642 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.086 | 0.305 |
| Number of reflections | 78552 | |
| <I/σ(I)> | 16.08 | 2.55 |
| Completeness [%] | 96.2 | 87 |
| Redundancy | 4.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 289 | 0.1 M Na/K phosphate pH 6.2, 10% PEG 3000, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
