3CZO
Crystal Structure of Double Mutant Phenylalanine Ammonia-Lyase From Anabaena Variabilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2007-02-08 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9183 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 195.153, 78.054, 156.758 |
| Unit cell angles | 90.00, 120.43, 90.00 |
Refinement procedure
| Resolution | 34.900 - 2.200 |
| R-factor | 0.21369 |
| Rwork | 0.210 |
| R-free | 0.27638 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nyn |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.501 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 134.800 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.070 | 0.390 |
| Number of reflections | 122951 | |
| <I/σ(I)> | 2.1 | |
| Completeness [%] | 97.0 | 92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 12-20% PEG1500 and 100 mM SPG (succinic acid, sodium dihydrogen phosphate, glycine), pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
