3CZN
Golgi alpha-mannosidase II (D204A nucleophile mutant) in complex with GnMan5Gn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-30 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.926, 109.576, 138.956 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.540 - 1.400 |
| R-factor | 0.178 |
| Rwork | 0.177 |
| R-free | 0.19700 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.215 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.143 | 0.494 |
| Number of reflections | 204675 | |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.2 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG8000, Tris, pH 7, 2.5% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
