3CZL
Crystal Structure Analysis of Sucrose hydrolase(SUH) E322Q-glucose complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.2399 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 96.800, 119.100, 55.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| Rwork | 0.203 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cze |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.054 | 0.143 |
| Number of reflections | 44083 | |
| <I/σ(I)> | 16.1 | |
| Completeness [%] | 99.0 | 91.2 |
| Redundancy | 6.8 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.1M HEPES, 0.2M calcium acetate, 16% PEG 3000, 5mM DTT, 0.1M cesium chloride , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
