3CZE
Crystal Structure Analysis of Sucrose hydrolase (SUH)- Tris complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97940, 0.97954, 0.97172 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 95.500, 117.000, 55.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| Rwork | 0.175 |
| R-free | 0.21500 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.172 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
| Rmerge | 0.110 | 0.066 | 0.591 |
| Number of reflections | 49685 | ||
| <I/σ(I)> | 7.3 | ||
| Completeness [%] | 99.7 | 99.8 | 99.2 |
| Redundancy | 9.6 | 9.6 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.1M HEPES, 0.2M calcium acetate, 16% PEG 3000, 5mM DTT, 0.1M guanidine chloride, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
