3CZ3
Crystal structure of Tomato Aspermy Virus 2b in complex with siRNA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2006-10-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97907, 0.97927, 0.96411 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 120.900, 165.670, 35.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.230 |
| R-factor | 0.231 |
| Rwork | 0.226 |
| R-free | 0.27400 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.467 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.090 | 0.574 |
| Number of reflections | 10597 | |
| <I/σ(I)> | 10.7 | 2.6 |
| Completeness [%] | 87.9 | 69.6 |
| Redundancy | 5.9 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 293 | 20% PEG 4000, 0.2 M ammonium sulfate, 0.1 M sodium acetate, pH 5.0, VAPOR DIFFUSION, temperature 293K |
| 1 | VAPOR DIFFUSION | 5 | 293 | 20% PEG 4000, 0.2 M ammonium sulfate, 0.1 M sodium acetate, pH 5.0, VAPOR DIFFUSION, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 1 | ammonium sulfate | ||
| 3 | 1 | 1 | sodium acetate | ||
| 4 | 1 | 2 | PEG 4000 | ||
| 5 | 1 | 2 | ammonium sulfate | ||
| 6 | 1 | 2 | sodium acetate |
