3CYQ
The crystal structure of the complex of the C-terminal domain of Helicobacter pylori MotB (residues 125-256) with N-acetylmuramic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.781, 110.058, 113.664 |
| Unit cell angles | 90.00, 104.58, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.300 |
| R-factor | 0.22 |
| Rwork | 0.217 |
| R-free | 0.28900 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.662 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 109.760 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.099 | 0.316 |
| Number of reflections | 103524 | |
| <I/σ(I)> | 7 | 2.8 |
| Completeness [%] | 96.0 | 98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 293 | 100 mM Tris/HCl, 16-18% PEG 3350, 200 mM sodium tartrate, 10 mM N-acetylmuramic acid, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
