3CXS
Crystal structure of human GNA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-15 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 50.133, 50.133, 142.611 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.241 |
| Rwork | 0.239 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2huz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.196 |
| Data scaling software | LSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.830 |
| High resolution limit [Å] | 2.700 | 5.410 | 2.700 |
| Number of reflections | 5121 | ||
| <I/σ(I)> | 8.96 | 17 | 2.1 |
| Completeness [%] | 94.5 | 94.1 | |
| Redundancy | 5.53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 289 | 0.2M MgCl2, 0.1M Bis-Tris pH 6.3, 25% w/v PEG 3350, vapor diffusion, hanging drop, temperature 289K |
