3CXB

Crystal Structure of sifa and skip

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Collection date	2006-10-28
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	91.690, 110.679, 45.533
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.600
Rwork	0.258
R-free	0.28770
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.490
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.048	0.472
Number of reflections	14191
<I/σ(I)>	14.4
Completeness [%]	95.3	99.9
Redundancy	14.3	14.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	20% Poly acrylic acid5100, 0.05M magnesium chloride, 0.1M Hepes, pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	20% Poly acrylic acid5100, 0.05M magnesium chloride, 0.1M Hepes, pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K