3CWQ
Crystal structure of chromosome partitioning protein (ParA) in complex with ADP from Synechocystis sp. Northeast Structural Genomics Consortium Target SgR89
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97898 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.609, 70.142, 112.209 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.620 - 2.470 |
| R-factor | 0.22368 |
| Rwork | 0.221 |
| R-free | 0.28173 |
| Structure solution method | SAD |
| RMSD bond length | 0.031 |
| RMSD bond angle | 2.669 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.620 | 2.540 |
| High resolution limit [Å] | 2.470 | 2.470 |
| Rmerge | 0.064 | 0.220 |
| Number of reflections | 33511 | |
| <I/σ(I)> | 32.74 | 6.96 |
| Completeness [%] | 97.8 | 90.7 |
| Redundancy | 7.1 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH UNDER OIL | 7.5 | 277 | Protein solution: 10 mM Tris-HCl pH 7.5, 100 Sodium chloride, 5 mM DTT. Resevoir solution: 25% Ethylene glycol, MICROBATCH UNDER OIL, temperature 277K |
