3CW6

E. coli Initiator tRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Collection date	2006-11-25
Wavelength(s)	0.931
Spacegroup name	P 64 2 2
Unit cell lengths	93.800, 93.800, 218.590
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.905 - 3.300
R-factor	0.235
Rwork	0.231
R-free	0.27400
RMSD bond length	0.009
RMSD bond angle	1.946
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.25)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.905	81.380	3.480
High resolution limit [Å]	3.300	10.440	3.300
Rmerge	0.103	0.043	0.674
Total number of observations		2453	4286
Number of reflections	7902
<I/σ(I)>	4.9	12.1	1.1
Completeness [%]	91.4	92.4	67.4
Redundancy	8	7.5	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	292	2.0 M Ammonium Sulphate, pH 8.0, VAPOR DIFFUSION, temperature 292K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	(NH4)2SO4
2	1	2	(NH4)2SO4