3CW4

Large c-terminal domain of influenza a virus RNA-dependent polymerase PB2

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-21
Detector	ADSC QUANTUM 210
Wavelength(s)	1.07172, 1.07204, 1.05382
Spacegroup name	P 32 2 1
Unit cell lengths	52.527, 52.527, 156.357
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.270 - 2.700
R-factor	0.23995
Rwork	0.237
R-free	0.29041
Structure solution method	MAD
RMSD bond length	0.025
RMSD bond angle	2.063
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	7328
Completeness [%]	98.8	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	2.2M formate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K