3CW4
Large c-terminal domain of influenza a virus RNA-dependent polymerase PB2
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-21 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.07172, 1.07204, 1.05382 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 52.527, 52.527, 156.357 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.270 - 2.700 |
| R-factor | 0.23995 |
| Rwork | 0.237 |
| R-free | 0.29041 |
| Structure solution method | MAD |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.063 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 7328 | |
| Completeness [%] | 98.8 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 2.2M formate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
