3CW0
E.coli DmsD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Collection date | 2008-01-05 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 97.449, 97.449, 210.042 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 88.400 - 2.400 |
| R-factor | 0.19675 |
| Rwork | 0.194 |
| R-free | 0.23968 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.414 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 97.590 | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
| Rmerge | 0.097 | 0.032 | 0.389 |
| Number of reflections | 55317 | ||
| <I/σ(I)> | 11.5 | ||
| Completeness [%] | 99.9 | 99.9 | 99.7 |
| Redundancy | 3.9 | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1M Ammonium sulphate, 0.1M HEPES, 0.5% PEG8K, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
