3CVY

Drosophila melanogaster (6-4) photolyase bound to repaired ds DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	90
Detector technology	CCD
Collection date	2007-11-19
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97925
Spacegroup name	P 21 21 21
Unit cell lengths	83.360, 88.840, 86.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.530 - 2.700
R-factor	0.21511
Rwork	0.212
R-free	0.26585
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.007
RMSD bond angle	1.112
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	CNS
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.087	0.338
Number of reflections	17459
<I/σ(I)>	13	4.8
Completeness [%]	95.8	90.2
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.6	293	20-25% PEG1500, 0.1 M MIB buffer, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	MIB buffer
2	1	2	MIB buffer
3	1	2	PEG1500