3CVP
Structure of Peroxisomal Targeting Signal 1 (PTS1) binding domain of Trypanosoma brucei Peroxin 5 (TbPEX5)complexed to PTS1 peptide (10-SKL)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-28 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.100, 66.277, 51.621 |
| Unit cell angles | 90.00, 104.70, 90.00 |
Refinement procedure
| Resolution | 32.950 - 2.000 |
| R-factor | 0.214 |
| Rwork | 0.212 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB code 1FCH |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.095 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.950 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 19221 | |
| <I/σ(I)> | 11.1 | 2.5 |
| Completeness [%] | 96.3 | 79.1 |
| Redundancy | 2.71 | 1.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 2.3M Potassium acetate, 0.1M sodium citrate monohydrate, pH 4.8 - 5.0, vapor diffusion, sitting drop, temperature 298K |
