3CVL
Structure of Peroxisomal Targeting Signal 1 (PTS1) binding domain of Trypanosoma brucei Peroxin 5 (TbPEX5)complexed to T. brucei Phosphofructokinase (PFK) PTS1 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-11-15 |
Detector | RIGAKU SATURN 944 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.390, 66.360, 49.680 |
Unit cell angles | 90.00, 105.13, 90.00 |
Refinement procedure
Resolution | 26.170 - 2.150 |
R-factor | 0.215 |
Rwork | 0.212 |
R-free | 0.27800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB code 1FCH |
RMSD bond length | 0.014 |
RMSD bond angle | 1.368 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.400 | 2.150 |
High resolution limit [Å] | 2.080 | 2.080 |
Number of reflections | 16165 | |
<I/σ(I)> | 6.5 | 2.1 |
Completeness [%] | 97.6 | 85.4 |
Redundancy | 3.27 | 2.73 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 298 | 2.3M Potassium acetate, 0.1M sodium citrate monohydrate, pH 4.8, VAPOR DIFFUSION, SITTING DROP, temperature 298K |