3CVG
Crystal structure of a periplasmic putative metal binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-14 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 150.782, 150.782, 116.687 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.140 - 1.970 |
| R-factor | 0.259 |
| Rwork | 0.259 |
| R-free | 0.28900 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | 0.600 |
| Number of reflections | 103728 | |
| <I/σ(I)> | 7.3 | 1 |
| Completeness [%] | 98.0 | 82.1 |
| Redundancy | 22.6 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | Bis-tris, PEG 3350, MgCl2, CaCl2, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
