3CUT
Crystal structure of glycogen phosphorylase b in complex with N-(-D-glucopyranosyl)-N'-(2-naphthyl)oxamide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX10.1 |
Synchrotron site | SRS |
Beamline | PX10.1 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2007-11-16 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.04498 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.712, 128.712, 116.819 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.590 - 2.300 |
R-factor | 0.19433 |
Rwork | 0.193 |
R-free | 0.22028 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1hlf |
RMSD bond length | 0.008 |
RMSD bond angle | 1.103 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 43315 | |
<I/σ(I)> | 7.9 | 3.7 |
Completeness [%] | 98.0 | 99 |
Redundancy | 5.2 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 6.7 | 287 | BES, EDTA, pH6.7, SMALL TUBES, temperature 287K |