3CUO
Crystal structure of the predicted DNA-binding transcriptional regulator from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.994, 39.632, 56.279 |
| Unit cell angles | 95.93, 95.78, 97.41 |
Refinement procedure
| Resolution | 30.530 - 2.000 |
| R-factor | 0.23051 |
| Rwork | 0.227 |
| R-free | 0.28875 |
| Structure solution method | SAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.849 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.640 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.093 | 0.261 |
| Number of reflections | 19625 | |
| <I/σ(I)> | 13.06 | 3 |
| Completeness [%] | 90.4 | 67.52 |
| Redundancy | 4.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 4M Na Formate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
