3CUL
Aminoacyl-tRNA synthetase ribozyme
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795, 0.9792, 1.2157 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 192.151, 48.725, 90.515 |
| Unit cell angles | 90.00, 93.50, 90.00 |
Refinement procedure
| Resolution | 29.570 - 2.800 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.28300 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.372 | |
| Number of reflections | 36041 | |
| <I/σ(I)> | 28.8 | 3.3 |
| Completeness [%] | 99.1 | 99.7 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 295 | 100 mM magnesium formate, 15% PEG 3000, 1 M lithium chloride, pH 7.0, VAPOR DIFFUSION, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | magnesium formate | ||
| 2 | 1 | 2 | magnesium formate | ||
| 3 | 1 | 2 | PEG 3000 | ||
| 4 | 1 | 2 | lithium chloride |
