3CU4
OmcF, Outer membrance cytochrome F from Geobacter sulfurreducens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-13 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.080, 40.190, 48.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.010 - 1.300 |
| R-factor | 0.169 |
| Rwork | 0.169 |
| R-free | 0.18300 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 2.100 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.310 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.037 | 0.105 |
| Number of reflections | 33541 | |
| <I/σ(I)> | 42.4 | 9.7 |
| Completeness [%] | 96.0 | 70 |
| Redundancy | 4.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 298 | 1.2 M trisodium citrate, 0.1 M Tris pH 8.5 (Salt Rx screen-22), microseeding, pH 5.0, VAPOR DIFFUSION, temperature 298K |
