3CTG
Crystal structure of reduced glutaredoxin 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 43 |
| Unit cell lengths | 41.630, 41.630, 78.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.130 - 1.500 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ctf |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | AUTOMAR |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.130 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.076 | 0.141 |
| Number of reflections | 20618 | |
| <I/σ(I)> | 6.4 | 3.3 |
| Completeness [%] | 98.5 | 99.4 |
| Redundancy | 2.91 | 2.97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 289 | 30% PEG 4000, 0.1M NAAC, 0.2M NH4AC, VAPOR DIFFUSION, HANGING DROP, pH 4.60, temperature 289K |
