3CTD
Crystal structure of a putative AAA family ATPase from Prochlorococcus marinus subsp. pastoris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-21 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 108.184, 108.184, 68.685 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.214 |
| Rwork | 0.211 |
| R-free | 0.27500 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.674 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.559 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.075 | 0.123 |
| Number of reflections | 14631 | |
| <I/σ(I)> | 22.7 | 12.5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 8 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 294 | 100mM Hepes pH 7.0, 2% PEG 3350, 240mM Tri-sodium citrate dihydrate pH 7.0, VAPOR DIFFUSION, temperature 294K |
