3CT0
Crystal and cryoEM structural studies of a cell wall degrading enzyme in the bacteriophage phi29 tail
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.876, 69.350, 70.728 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.050 - 1.770 |
| R-factor | 0.19934 |
| Rwork | 0.195 |
| R-free | 0.23811 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.544 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.100 | 1.830 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.035 | 0.171 |
| Number of reflections | 14874 | |
| <I/σ(I)> | 35.6 | 9.9 |
| Completeness [%] | 97.4 | 89.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 20% PEG4000, 100mM Tris-HCl, 10% glycerol, 10mM NAG4, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
