3CRZ
Ferredoxin-NADP Reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-05-22 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 67.140, 73.490, 51.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| Rwork | 0.181 |
| R-free | 0.21800 |
| Starting model (for MR) | 2qdx |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.700 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.038 | 0.157 |
| Number of reflections | 20651 | |
| <I/σ(I)> | 28.45 | 8.44 |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 6.61 | 6.47 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 290 | ammonium acetate, PEG4000, pH 5.6, vapor diffusion, hanging drop, temperature 290K |
