3CRY
Gamma-glutamyl cyclotransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.95361 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.828, 98.135, 100.499 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.370 - 1.700 |
| R-factor | 0.17983 |
| Rwork | 0.178 |
| R-free | 0.22313 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rbh |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.241 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.190 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.092 | 0.419 |
| Number of reflections | 38628 | |
| <I/σ(I)> | 12.6 | 3.7 |
| Completeness [%] | 99.4 | 97.7 |
| Redundancy | 4.6 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 50% PEG 4000, 0.2M ammonium acetate, 0.1M sodium acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
