3CRP
A heterospecific leucine zipper tetramer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-05-18 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 79.473, 79.473, 54.947 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.100 - 1.700 |
| R-factor | 0.20419 |
| Rwork | 0.203 |
| R-free | 0.23045 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nrn |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.534 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.100 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.055 | 0.556 |
| Number of reflections | 19756 | |
| <I/σ(I)> | 15.2 | 4.9 |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 9.2 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.8 | 295 | 0.1M Tris-HCl, 0.05M magnesium chloride, 13% PEG 4000, pH 9.8, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
