3CRO
THE PHAGE 434 CRO/OR1 COMPLEX AT 2.5 ANGSTROMS RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-13 |
| Temperature [K] | 277 |
| Detector technology | AREA DETECTOR |
| Detector | XENTRONICS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.200, 47.600, 61.700 |
| Unit cell angles | 90.00, 109.50, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| R-factor | 0.22 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.300 |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.101 * |
| Number of reflections | 7232 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.2 | 4 * | pH 6.20, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | PEG 3500 | ||
| 3 | 1 | 1 | MES | ||
| 4 | 1 | 1 | NACL | ||
| 5 | 1 | 1 | MGCL2 | ||
| 6 | 1 | 1 | SPERMINE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 2 | PEG3500 | 12 (%(w/v)) | |
| 2 | 1 | 2 | MES | 100 (mM) | pH6.2 |
| 3 | 1 | 2 | 160 (mM) | ||
| 4 | 1 | 2 | 120 (mM) | ||
| 5 | 1 | 2 | spermine | 2 (mM) | |
| 6 | 1 | protein | 2 (mM) | ||
| 7 | 1 | 1 | DNA | 1 (mM) |
