3CRN
Crystal structure of response regulator receiver domain protein (CheY-like) from Methanospirillum hungatei JF-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2007-02-20 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.930, 53.992, 92.681 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.880 - 1.580 |
| R-factor | 0.18516 |
| Rwork | 0.183 |
| R-free | 0.22389 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.264 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.700 | 1.640 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.059 | 0.950 |
| Number of reflections | 36387 | |
| <I/σ(I)> | 10.3 | 0.3 |
| Completeness [%] | 97.2 | 92.1 |
| Redundancy | 6.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 2.4M Sodium malonate pH 7.0, 10% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
