3CRM
Structure of tRNA Dimethylallyltransferase: RNA Modification through a Channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-15 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 41 |
| Unit cell lengths | 76.083, 76.083, 58.302 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| Rwork | 0.211 |
| R-free | 0.23000 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.150 |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 25202 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 278 | PEG 6000, pH 8.5, vapor diffusion, hanging drop, temperature 278K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 278 | PEG 6000, pH 8.5, vapor diffusion, hanging drop, temperature 278K |
