3CRK
Crystal structure of the PDHK2-L2 complex.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.380, 120.679, 71.466 |
| Unit cell angles | 90.00, 96.03, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.21 |
| Rwork | 0.208 |
| R-free | 0.25060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.009 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | COMO |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.070 | 2.300 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.055 | 0.269 |
| Number of reflections | 50377 | |
| <I/σ(I)> | 18.8 | 3.2 |
| Completeness [%] | 94.9 | 73.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 293 | 0.1 M sodium acetate (pH 5.3) and 0.5 M sodium formate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
