3CRJ
Crystal structure of a TetR transcription regulator from Haloarcula marismortui ATCC 43049
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-13 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.494, 103.361, 167.898 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.990 - 2.600 |
| R-factor | 0.23628 |
| Rwork | 0.234 |
| R-free | 0.28789 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.445 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.990 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.116 | 0.606 |
| Number of reflections | 32786 | |
| <I/σ(I)> | 18.76 | 1.35 |
| Completeness [%] | 96.3 | 72.1 |
| Redundancy | 4.9 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.15M DL-malic acid, 20% w/v PEG 3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
