3CRA

Crystal Structure of Escherichia coli MazG, the Regulator of Nutritional Stress Response

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6C1
Synchrotron site	PAL/PLS
Beamline	6C1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-22
Detector	ADSC QUANTUM 210
Wavelength(s)	1.23985
Spacegroup name	P 21 21 21
Unit cell lengths	62.789, 67.568, 140.425
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.470 - 2.100
R-factor	0.22924
Rwork	0.226
R-free	0.28394
Structure solution method	SAD
RMSD bond length	0.024
RMSD bond angle	2.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.038
Number of reflections	41095

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	0.1M trisodium citrate(pH 5.5), 0.2M ammonium acetate, 20% polyethylene glycol 4000, VAPOR DIFFUSION, SITTING DROP, temperature 298K