3CR6
Crystal Structure of the R132K:R111L:A32E Mutant of Cellular Retinoic Acid Binding Protein Type II Complexed with C15-aldehyde (a retinal analog) at 1.22 Angstrom resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97869 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.852, 46.347, 37.324 |
| Unit cell angles | 90.00, 92.53, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.220 |
| R-factor | 0.124 |
| Rwork | 0.114 |
| R-free | 0.18480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g7b |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.031 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.070 |
| High resolution limit [Å] | 1.030 | 1.030 |
| Rmerge | 0.046 | 0.806 |
| Number of reflections | 42691 | |
| <I/σ(I)> | 9.5 | 0.759 |
| Completeness [%] | 72.4 | 4.8 |
| Redundancy | 3.8 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1M sodium citrate, 0.2M ammonium acetate, 26% PEG 4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
